An improved method for proteomics studies in C. elegans by fluorogenic derivatization, HPLC isolation, enzymatic digestion and liquid chromatography--tandem mass spectrometric identification |
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| Authors: | Masuda Mayumi Saimaru Hiroshi Takamura Norio Imai Kazuhiro |
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| Affiliation: | Laboratory of Bio-Analytical Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. |
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| Abstract: | An improved method for proteomics studies, which includes the fluorogenic derivertization of protein mixtures with 7-chloro-4-(dimethylaminoethylaminosulfonyl)-2,1,3-benzoxadiazole (DAABD-Cl), followed by HPLC isolation, enzymatic digestion and identification of the derivatized proteins by HPLC-electrospray ionization (ESI)-MS/MS with the probability-based protein identification algorithm, identified 103 proteins in the soluble extract (10 microg protein) of Caenorhabditis elegans. |
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| Keywords: | 7‐chloro‐4‐(dimethylaminoethylaminosulfonyl)‐2,1,3‐benzoxadiazole fluorescent derivatization ESI‐MS/MS proteomics Caenorhabditis elegans |
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