| 高速逆流法对刺五加有效成分刺五加苷E的分离制备 |
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| 引用本文： | 魏芸,张天佑,吴克友.高速逆流法对刺五加有效成分刺五加苷E的分离制备[J].色谱,2002,20(6):543-545. |
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| 作者姓名： | 魏芸 张天佑 吴克友 |
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| 作者单位： | 1. 北京市新技术应用研究所,北京天然产物分离纯化技术研究中心,北京,100035
2. 北京理工大学化学系,北京,100081 |
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| 摘 要： | 采用高速逆流法分离纯化刺五加粗品中的刺五加苷E。溶剂系统为氯仿 甲醇 异丙醇 水,体积比为5∶6∶1∶4,上相为固定相,下相为流动相,转速为800r/min,流速为2 0mL/min。所得刺五加苷E经高效液相分析,测定纯度为98%(峰面积百分比)。这为其他苷类等极性物质的分离纯化提供了依据。
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| 关 键 词： | 高速逆流法 分离 制备 刺五加苷 刺五加 |
| 文章编号： | 1000-8713(2002)06-0543-03 |
| 修稿时间： | 2002年3月14日 |
Separation of Eleutheroside E from Crude Extract of Radix Acanthopanacis Senticosus by Analytical and Preparative High-Speed Countercurrent Chromatography |
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| Yun Wei,Tian-you Zhang,Ke-you Wu.Separation of Eleutheroside E from Crude Extract of Radix Acanthopanacis Senticosus by Analytical and Preparative High-Speed Countercurrent Chromatography[J].Chinese Journal of Chromatography,2002,20(6):543-545. |
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| Authors: | Yun Wei Tian-you Zhang Ke-you Wu |
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| Institution: | Beijing Research Center for Separation & Purification Technology of Natural Products, Beijing Institute of New Technology Application, Beijing 100035, China. weiyun0570@sina.com |
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| Abstract: | Analytical high-speed countercurrent chromatography (HSCCC) was used for the systematic optimization of the two-phase solvent system to separate eleutheroside E from crude extract of Radix Acanthopanacis Senticosus. Eleutheroside E was obtained by preparative HSCCC with two-phase solvent system composed of chloroform-methanol-isopropanol-water (5:6:1:4 in volume ratio). The mobile phase is the lower phase and operated at a flow-rate of 2.0 mL/min, while the apparatus rotated at 800 r/min. High performance liquid chromatographic analysis of eleutheroside E revealed that its purity was over 98%. HSCCC is a useful method for the separation of natural products. |
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| Keywords: | high-speed countercurrent chromatography separation preparation eleutheroside E Radix Acanthopanacis Senticosus |
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