首页 | 本学科首页   官方微博 | 高级检索  
     


LC-ESI-MS Determination of Bilobalide and Ginkgolides in Canine Plasma
Authors:Jingling Tang  Jin Sun  Yinghua Sun  Fude Cui  Zhonggui He
Affiliation:(1) Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China
Abstract:
A sensitive and selective method using liquid chromatography with electrospray ionization mass spectrometric detection was developed for the quantification of bilobalide and ginkgolides in canine plasma. The analytes were extracted with diethyl ether-dichloromethane-isopropanol (6:3:1, v/v) after spiking the samples with daidzein (internal standard). The lower limit of quantification (LLOQ) of the method was 2.5 µg L?1 for ginkgolide B and 10.0 µg L?1 for bilabolide, ginkgolide A and ginkgolide C. The accuracy of the method was within 15% of the actual values over a wide range of plasma concentrations. The intra-day and inter-day precision was better than 15% (R.S.D.). Finally, the LC-ESI-MS method was successfully applied to study the pharmacokinetics of ginkgolides and bilabolide after administration of Ginkgo biloba extracts to dogs.
Keywords:Column liquid chromatography  LC-ESI-MS  Pharmacokinetics  Bilabolide and ginkgolides in canine plasma
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号