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Low flow rate modifier addition in packed capillary column supercritical fluid chromatography
Authors:Elena Iba  ez,Wenbao Li,Abdul Malik,Milton L. Lee
Affiliation:Elena Ibañez,Wenbao Li,Abdul Malik,Milton L. Lee
Abstract:
A new method to accurately deliver small amounts (0.5 to 20 mol%) of modifier into CO2 was used to study the effects of three different modifiers (methanol, water, and formic acid) in packed capillary column SFC. The method allows the use of different modifiers, with minimal instrument modification. The effects of the different modifiers at different concentrations on retention and peak shape are shown by analyzing a polarity test mixture and a sample of free fatty acids.
Keywords:Supercritical fluid chromatography  Modifier addition  Packed capillary columns
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