Separation of tetracycline and its related substances by capillary zone electrophoresis |
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Authors: | Ann Van Schepdael Jan Saevels Xavier Lepoudre Rukia Kibaya Ni Zhi Gang Eugn Roets Jos Hoogmartens |
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Institution: | Ann Van Schepdael,Jan Saevels,Xavier Lepoudre,Rukia Kibaya,Ni Zhi Gang,Eugèn Roets,Jos Hoogmartens |
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Abstract: | Tetracycline was separated from its main impurities 4-epitetracycline, anhydrotetracycline, 4-epianhydrotetracycline, chlortetracycline, and demethyltetracycline by capillary electrophoresis. Systematic method development was performed in which following parameters were consecutively optimized: type and pH of the buffer, buffer concentration, type and concentration of organic modifier, voltage and temperature. Qualitative and quantitative aspects of the method are compared with liquid chromatography. |
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Keywords: | Capillary electrophoresis Tetracycline Antibiotics Related substances |
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