| Expression of Catalytic Domain of Protein Tyrosine Phosphatase 1B and Preparation of Its Polyclonal Antibody |
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| 作者姓名： | SHI Dong-lei DONG Hong-bo ZHU Zhi-cheng SUN Mei SU Ji-quan ZHANG Xing-yi XU Yue-chi FU Xue-qi |
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| 作者单位： | SHI Dong-lei1，DONG Hong-bo2，ZHU Zhi-cheng1，SUN Mei3，SU Ji-quan1，ZHANG Xing-yi1，XU Yue-chi4 and FU Xue-qi41. Department of Thoracic Surgery，The Second Hospital of Jilin University 2. College of Pharmacy; 3. Department of Pathology，The Second Hospital of Jilin University; 4. College of Life Sciences，Jilin University，Changchun 130012，P. R. China |
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| 摘 要： | This study focuses on the expression of human protein tyrosine phosphatase 1B(PTP1B) catalytic domain (△PTP1B) and preparation of polyclonal antibody against △PTP1B. △PTP1B gene was PCR amplified with the cDNA of human PTP1B as the template, and cloned into the pT7 expression vector. The recombinant pT7-△PTP1B was expressed in E. coli Rosetta( DE3 ) host cells and purified. The antiserum was prepared by immunizing rabbit with purified recombinant △PTP1B. The polyclonal antibody against △PTP1B was purified by PVDF immobilized antigen affinity chromatography. △PTP1B was correctly cloned, expressed, and purified as confirmed by PCR, DNA sequence ratio) and 0. 1 ng, respectively. This study provides an important basis for further studying the biological function of PTP1B and its relationship with human diseases.
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| 关 键 词： | 蛋白酪氨酸磷酸酶1B 催化活性结构域 多克隆抗体 制备 表达 |
| 收稿时间： | 2006-12-22 |
Expression of Catalytic Domain of Protein Tyrosine Phosphatase 1B and Preparation of Its Polyclonal Antibody |
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| SHI Dong-lei DONG Hong-bo ZHU Zhi-cheng SUN Mei SU Ji-quan ZHANG Xing-yi XU Yue-chi FU Xue-qi.Expression of Catalytic Domain of Protein Tyrosine Phosphatase 1B and Preparation of Its Polyclonal Antibody[J].Chemical Research in Chinese University,2007,23(2):204-207. |
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| Authors: | Dong-lei SHI Hong-bo DONG Zhi-cheng ZHU Mei SUN Ji-quan SU Xing-yi ZHANG Yue-chi XU Xue-qi FU |
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| Institution: | aDepartment of Thoracic Surgery, The Second Hospital of Jilin University, Changchun 130012, P. R. China;bCollege of Pharmacy, Jilin University, Changchun 130012, P. R. China;cDepartment of Pathology, The Second Hospital of Jilin-University, Changchun 130012, P. R. China;dCollege of Life Sciences, Jilin University, Changchun 130012, P. R. China |
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| Abstract: | |
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| Keywords: | Protein tyrosine phosphatase 1 B Catalytic domain Polyclonal antibody |
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