Determination of H2O2 and organic peroxides by high-performance liquid chromatography with post-column UV irradiation, derivatization and fluorescence detection |
| |
| Authors: | Jianguo Hong J Maguhn Dieter Freitag Antonius Kettrup |
| |
| Institution: | GSF-Institut für ?kologische Chemie, Schulstrasse 10, D-85356 Freising, Germany e-mail: maguhn@gsf.de, DE
|
| |
| Abstract: | A method for the determination of hydrogen peroxide and several organic peroxides by high-performance liquid chromatography
with post-column UV irradiation, derivatization and fluorescence detection is described. By means of post-column UV irradiation
in the presence of water organic peroxides are converted into hydrogen peroxide and organic hydroperoxides, which react rapidly
with the post-column derivatization agent p-hydroxyphenylacetic acid (PHPAA) under catalysis of horseradish peroxidase to
yield the fluorescent PHPAA dimer that is detected at excitation and emission wavelengths of 285 and 400 nm, respectively.
The detection limit for hydrogen peroxide is 14 ng/mL, for organic peroxides between 34 ng/mL and 5 μg/mL. No interference
by other compounds was observed when their concentrations were below 10 mg/mL except ethers and phenols.
Received: 6 August 1997 / Revised: 11 December 1997 / Accepted: 15 December 1997 |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
