On Penicillin‐Binding Protein 1b Affinity‐Labeling Reagents |
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Authors: | Daniela Volke Mohammed Daghish Lothar Hennig Matthias Findeisen Sabine Giesa Ramona Oehme Peter Welzel |
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Institution: | Universität Leipzig, Fakultät für Chemie und Mineralogie, Johannisallee 29, D-04103 Leipzig |
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Abstract: | The synthesis of some 3‐aryl‐3‐(trifluoromethyl)3H‐diazirine and benzophenone‐based photoaffinity labels is reported. The photolabile group is bound to a scaffold that also accommodates functional groups to which an indicator unit (biotin) and the bioactive ligand can be attached orthogonally. To three of the labels, moenomycin was conjugated with the aim to provide tools for the identification of the moenomycin binding site within the transglycosylase domain of the enzyme PBP 1b. Some preliminary photoaffinity‐labeling experiments were carried out. |
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