| 青海省唐古特铁线莲中齐墩果酸含量的测定 |
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| 引用本文： | 苏旭,刘玉萍,杨淳彬,吴学明.青海省唐古特铁线莲中齐墩果酸含量的测定[J].分析试验室,2012(5):18-21. |
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| 作者姓名： | 苏旭 刘玉萍 杨淳彬 吴学明 |
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| 作者单位： | 青海师范大学生命与地理科学学院;青海师范大学青藏高原环境与资源教育部重点实验室;青海师范大学思想政治理论课教学科研部 |
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| 基金项目： | 教育部“春晖计划”合作科研项目(Z2010078);青海省自然科学基金项目(2011-Z-745);青海师范大学科研创新计划项目;青海师范大学博士科研启动经费(202500205);国家自然科学基金项目(30370110)资助 |
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| 摘 要： | 建立了唐古特铁线莲中齐墩果酸含量测定的HPLC方法。色谱条件:采用Phenomenex Luna C18(250 mm×4.6 mm,5μm),流动相:乙腈-0.2%H3PO4(35:65),流速:1 mL/min,柱温:室温(25℃),检测波长:205 nm。齐墩果酸在0.0233～0.7000 mg范围内呈良好的线性关系,线性回归方程为y=2E+06x+360803,R2=0.9917,回收率RSD为1.7%,并对青海省野生和栽培唐古特铁线莲中齐墩果酸进行了定量分析,结果显示前者平均值为0.1184%,后者平均值为0.0651%。方法可用以测定唐古特铁线莲中齐墩果酸的含量。
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| 关 键 词： | 青海省 唐古特铁线莲 齐墩果酸 |
Determination of the oleanolic acid contents in clematis tangutica(Maxim.) Korsh from qinghai province |
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| SU Xu,LIU Yu-ping,YANG Chun-bin,and WU Xue-ming.Determination of the oleanolic acid contents in clematis tangutica(Maxim.) Korsh from qinghai province[J].Chinese Journal of Analysis Laboratory,2012(5):18-21. |
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| Authors: | SU Xu LIU Yu-ping YANG Chun-bin and WU Xue-ming |
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| Institution: | 1(1.College of Geography and Life Science,Qinghai Normal University Xining 810008;2.Key Laboratory of Education Ministry on Environments and Resources in Tibetan Plateau,Qinghai Normal University Xining 810008;3.Department of Ideological and Political Theory Teaching and Research,Qinghai Normal University,Xining 810008), |
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| Abstract: | A HPLC method for the determination of oleanolic acid contents in clematis tangutica(maxim.) korsh was established in this article.Chromatographic conditions were as follows: adopting a phenomenex luna C18 cdumn(250 mm×4.6 mm,5 μm),mobile phase: ethanenitrile-0.2 % H3PO4 water(35:65),flow rate: 1 mL/min,The column temperature was the room temperature(25 ℃),detecttion wave length 205 nm.Linear rang of the concentration was 0.0233-0.7000 mg and the linear regression equation: y=2E+06x+360803,R2=0.9917,and relative standard deviation was 1.7 %.Meanwhile,the oleanolic acid in wild and cultural clematis tangutica(maxim.) korsh from Qinghai province was quantitatively analyzed.The results showed that the oleanolic acid content of the wild samples was 0.1184 %,and the cultural sample was 0.0651 %.It can be used for the determination of the oleanolic acid content in clematis tangutica(maxim.) korsh. |
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| Keywords: | Qinghai province Clematis tangutica(maxim ) korsh Oleanolic acid |
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