Electrofusion of protoplasts modified by protein adsorption |
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| Institution: | 1. Dept. of Physics, Nankai University, Tianjin 300071, China;2. Laboratory Bioelectrochemistry, Jena, Saxonian Academy of Sciences at Leipzig, Germany;1. Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, M5S 3E5, Canada;2. Department of Chemistry, University of Toronto, Toronto, ON, M5S 3H6, Canada;1. Department of Biomedical Sciences, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong SAR, People''s Republic of China;2. Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institutes of City University of Hong Kong, Shenzhen, People''s Republic of China;3. Key Laboratory of Sensing Technology and Biomedical Instruments (Guangdong Province), School of Engineering, Sun Yat-Sen University, Guangzhou, People''s Republic of China;4. Institute of Chinese Medical Sciences, University of Macau, Macau SAR, People''s Republic of China;5. Jinzhou Medical University, Liaoning, People''s Republic of China;1. Division of Thermal and Fluids Science, Institute for Computational Science, Faculty of Electrical and Electronics Engineering, Ton Duc Thang University, Ho Chi Minh City, Vietnam;2. Department of Mechanical Engineering, Incheon National University, Incheon, Republic of Korea;3. Department of Biomedical Engineering, National Cheng Kung University, Taiwan;4. Center for Micro/Nano Science and Technology, National Cheng Kung University, Taiwan |
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| Abstract: | The synergistic influence of proteins at lowest concentrations (below mg ml−1) on barley protoplast electrofusion together with dielectrophoresis for adhesion of protoplast membranes has been studied by determination of the relative electrofusion yield Fr, which allows further insight into `protein modified electroporation and electrofusion'. As a general rule all proteins with isoelectric points pI>8 stimulate fusion: Fr>1 in buffer free mannitol solution (pH 6.5) reaching levels at Fr=2∼3. Proteins with pI<8 show at lowest concentrations (<1 mg ml−1) also a stimulating effect: Fr>1 followed by the inhibition of Fr—sometimes reaching zero—accompanied by rising conductivity with increasing concentrations. These results are important for resealing, insertion, transfection and the role of integral membrane proteins for the mechanism of fusion. |
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