| 光散射法快速灵敏测定和表征啤酒酵母 |
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| 引用本文： | 陈绍芬,黄承志,谭克俊.光散射法快速灵敏测定和表征啤酒酵母[J].高等学校化学学报,2006,27(6):1023-1027. |
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| 作者姓名： | 陈绍芬 黄承志 谭克俊 |
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| 作者单位： | 西南大学化学化工学院,重庆,400715 |
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| 基金项目： | 国家自然科学基金(批准号30570465,20425517)，重庆市科委自然基金项目资助 |
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| 摘 要： | 研究了啤酒酵母的光散射光谱. 结果表明， 在波长308.0 nm处, 光散射强度与啤酒酵母浓度在2.0×104-2.0×106 Cell/mL 范围内呈线性关系, 检出限(3σ)为4.94×102 Cell/mL. 将此方法成功地应用于培养液中啤酒酵母含量的快速、 灵敏的测定, 并对其散射光谱进行了表征.
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| 关 键 词： | 光散射分析法 散射截面 吸收截面 啤酒酵母 生物量 |
| 文章编号： | 0251-0790（2006）06-1023-05 |
| 收稿时间： | 11 20 2005 12:00AM |
| 修稿时间： | 2005-11-20 |
Rapid and Sensitive Detection and Characterization of Saccharomyces cerevisiae by Light Scattering Method |
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| CHEN Shao-Fen,HUANG Cheng-Zhi,TAN Ke-Jun.Rapid and Sensitive Detection and Characterization of Saccharomyces cerevisiae by Light Scattering Method[J].Chemical Research In Chinese Universities,2006,27(6):1023-1027. |
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| Authors: | CHEN Shao-Fen HUANG Cheng-Zhi TAN Ke-Jun |
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| Institution: | College of Chemistry and Chemical Engineering, Southwest Normal University,
Chongqing 400715, China |
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| Abstract: | The light scattering(LS) spectra of Saccharomyces cerevisiae was studied. The results show that the LS intensities were in proportion to the concentrations of Saccharomyces cerevisiae at peak 308.0 nm in the concentration range of 2.0×104-2.0×106 Cell/mL. The detection limit(3σ) was 4.94x102 Cell/mL. The present new method was successfully applied to the rapid, sensitive determination and characterization of Saccharomyces cerevisiae biomass in suspensions. |
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| Keywords: | Light scattering assay Scattering cross section Absorption cross section Saccharomyces cerevisiae Biomass |
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