Robust and simple interface for microchip electrophoresis-mass spectrometry |
| |
Authors: | Tachibana Yuji Otsuka Koji Terabe Shigeru Arai Akihiro Suzuki Koichi Nakamura Shin |
| |
Institution: | Department of Material Science, Graduate School of Science, Himeji Institute of Technology, 3-2-1 Kouto, Kamigori, Hyogo 678-1297, Japan. ytac@sci.himeji-tech.ac.jp |
| |
Abstract: | A robust and simple interface for microchip electrophoresis-mass spectrometry (MCE-MS) was developed using a spray nozzle connected to the exit of the separation channel of the microchip. The spray nozzle was attached to the microchip using a polyether ether ketone screw without adhesive, thus allowing easy replaced. Sample injection and electrophoretic separation was performed by control of the voltage only. The analysis of a few basic drugs was performed using the optimized MCE-MS system. The separation was improved by using a high-viscosity separation buffer and a spray nozzle with a small bore size. This system was also applied to the separation of peptides and protein-trypsin digests. Sample adsorption was minimized by adding acetonitrile to the separation buffer when using a quartz microchip. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|