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Studies of interaction between colchicine and bovine serum albumin by fluorescence quenching method |
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| Authors: | Yan-Jun Hu Yi Liu Li-Xia Zhang Ru-Ming Zhao Song-Sheng Qu |
| Affiliation: | aDepartment of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, People's Republic of China bState Key Laboratory of Virology, Wuhan University, Wuhan 430072, People's Republic of China |
| Abstract: | We investigated the interaction between colchicine and bovine serum albumin (BSA) by fluorescence and UV–Vis absorption spectroscopy. In the mechanism discussion, it was proved that the fluorescence quenching of BSA by colchicine is a result of the formation of colchicine–BSA complex; van der Waals interactions and hydrogen bonds play a major role in stabilizing the complex. The modified Stern–Volmer quenching constant Ka and corresponding thermodynamic parameters ΔH, ΔG, ΔS at different temperatures were calculated. The distance r between donor (BSA) and acceptor (colchicine) was obtained according to fluorescence resonance energy transfer (FRET). |
| Keywords: | Colchicine Bovine serum albumin Fluorescence quenching Thermodynamic parameters Fluorescence resonance energy transfer |
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