Silica-nanoparticle-based interface for the enhanced immobilization and sequence-specific detection of DNA |
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Authors: | D Zhang Y Chen H -Y Chen X H Xia |
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Institution: | (1) Key Laboratory of Life Analytical Chemistry, Department of Chemistry, Nanjing University, Nanjing, 210093, China |
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Abstract: | A biocompatible and uniform interface based on silica nanoparticles derivatized with amino groups has been constructed for the effective immobilization and sensitive sequence-specific detection of calf thymus DNA. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) results showed that a monolayer of silica nanoparticles can be formed on a gold electrode under our experimental conditions using cysteine self-assembly monolayer as binder medium. Electrochemical impedance spectroscopy and X-ray photoelectron spectroscopy (XPS) verified the successful immobilization of DNA on silica-nanoparticle-modified gold electrodes. Quantitative results demonstrated that enhanced immobilization of single-strand DNA (ss-DNA) up to 1.6×10–8 mol cm–2 could be achieved owing to the larger surface area and the special properties of silica nanoparticles. In addition, hybridization experiments demonstrated that the immobilized ss-DNA on silica nanoparticles could specifically interact with complementary DNA in solutions. |
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Keywords: | Silica nanoparticles DNA immobilization Sequence-specific electrochemical detection |
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