A microfluidic SELEX prototype |
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Authors: | Glen Hybarger Joseph Bynum Robert F Williams James J Valdes James P Chambers |
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Institution: | (1) Department of Biology, The University of Texas at San Antonio, 6900 N. Loop 1604 W., San Antonio, TX 78249, USA;(2) US Army RDECOM ECBC, AMSSB-RRT, Bldg. E3330, Rm 143, Aberdeen Proving Ground, MD 21010-5424, USA |
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Abstract: | Aptamers are nucleic acid binding species capable of recognizing a wide variety of targets ranging from small organic molecules
to supramolecular structures, including organisms. They are isolated from combinatorial libraries of synthetic nucleic acid
by an iterative process referred to as SELEX (Systematic Evolution of Ligands by Exponential Enrichment). Here we describe
an automated microfluidic, microline-based assembly that uses LabView-controlled actuatable valves and a PCR machine, and
which is capable of the selection and synthesis of an anti-lysozyme aptamer as verified by sequence analysis. The microfluidic
prototype described is 1) a simple apparatus that is relatively inexpensive to assemble, making automated aptamer selection
accessible to many investigators, and 2) useful for the continued “morphing” of macro→meso→microfabricated structures until
a convergence to a few functional systems evolves and emerges, partly or completely achieving simpler, smaller and more rapid
SELEX applications. |
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Keywords: | SELEX Microfluidic Miniaturized Aptamer Prototype |
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