| A Study of Protein and Glycoprotein Syntheses in Pre-implantation Mouse Embryos Using Mini-2D-Electrophoresis, Video Densitometer Scanning and Computer-Image Analysis |
| |
| 作者姓名： | 郑明 曹詠清 B.Lofts |
| |
| 作者单位： | Department of Endocrinology and Laboratory of Reproductive Biology，Institute of Zoology，Academia Sinica，Beijing 100080 PRC，Department of Endocrinology and Laboratory of Reproductive Biology，Institute of Zoology，Academia Sinica，Beijing 100080 PRC，Department of Zoology，University of Hong Kong，Hong Kong |
| |
| 基金项目： | Project supported by a research grant from the Croucher Foundation on Embryo Biotechnology. |
| |
| 摘 要： | In the present study, proteins and glycoproteins of mouse embryos at 2-cell,morula and blastocyst stages were analyzed.The techniques of ~(35)S-Met incorporation,ConA antiserum-precipitating ConA-binding proteins, mini-2D-electrophoresis, fluorography,video densitometer scanning and the computer-lmage system were used for analyses.Results of the investigations indicated that proteins and glycoproteins were synthesized by the embryos in a gradual increase manner from 2-cell to blastocyst. A relatively large amount of glycoproteins was synthesized during the morula and blastocyst stages.Two specific small glycoproteins respectively with molecular weights about 6500 and 9000 and PIs at 5.0 and 6.5 were apparently synthesized in the blastocyst but not in the 2-cell or the morula.
|
A Study of Protein and Glycoprotein Syntheses in Pre-implantation Mouse Embryos Using Mini-2D-Electrophoresis, Video Densitometer Scanning and Computer-Image Analysis |
| |
| ZHENG Ming CAO Yong-Qing.A Study of Protein and Glycoprotein Syntheses in Pre-implantation Mouse Embryos Using Mini-2D-Electrophoresis, Video Densitometer Scanning and Computer-Image Analysis[J].Science in China(Chemistry),1993(1). |
| |
| Authors: | ZHENG Ming CAO Yong-Qing |
| |
| Abstract: | In the present study, proteins and glycoproteins of mouse embryos at 2-cell,morula and blastocyst stages were analyzed.The techniques of ~(35)S-Met incorporation,ConA antiserum-precipitating ConA-binding proteins, mini-2D-electrophoresis, fluorography,video densitometer scanning and the computer-lmage system were used for analyses.Results of the investigations indicated that proteins and glycoproteins were synthesized by the embryos in a gradual increase manner from 2-cell to blastocyst. A relatively large amount of glycoproteins was synthesized during the morula and blastocyst stages.Two specific small glycoproteins respectively with molecular weights about 6500 and 9000 and PIs at 5.0 and 6.5 were apparently synthesized in the blastocyst but not in the 2-cell or the morula. |
| |
| Keywords: | implantation mouse embryos glyeoproteins mini 2D-electrophoresis computer-image analysis |
| 本文献已被 CNKI 等数据库收录！ |
