| 热敏相分离荧光免疫分析乙肝表面抗原的新方法 |
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| 引用本文： | 朱庆枝,杨黄浩,李东辉,许金钩,张毅,张长弓.热敏相分离荧光免疫分析乙肝表面抗原的新方法[J].高等学校化学学报,1999,20(4):544-548. |
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| 作者姓名： | 朱庆枝 杨黄浩 李东辉 许金钩 张毅 张长弓 |
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| 作者单位： | 1. 教育部材料和生命过程分析科学开放实验室, 厦门大学化学系, 厦门 361005;
2. 教育部材料和生命过程分析科学开放实验室, 厦门大学抗癌研究中心, 厦门 361005 |
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| 基金项目： | 教育部博士点基金,福建省自然科学基金 |
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| 摘 要： | 以N-异丙基丙烯酰胺为单体,将水溶性热敏高分子聚异丙基丙烯酰胺(PNIP)和抗体偶联,用异硫氰酸荧光素标记羊抗人乙肝表面抗原抗体,建立了夹心型热敏相分离荧光免疫分析乙肝表面抗原(HBsAg)的新方法.评价了抗体在PNIP上的固定效率和非特异性吸附情况.HBsAg在0.5～100μg/mL范围内与体系荧光强度呈良好的线性关系,检出限为10ng/mLHBsAg.该方法既具有均相免疫分析的快速性,又有固相免疫分析的高灵敏度.用于乙肝病人血清中HBsAg水平的测定,结果令人满意.
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| 关 键 词： | 聚异丙基丙烯酰胺 热敏相分离 荧光免疫分析 乙肝表面抗原 |
| 收稿时间： | 1998-10-28 |
Fluorescence Immunoassay for HBsAg Using a Thermal Phase Separating Polymer |
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| ZHU Qing-Zhi,YANG Huang-Hao,LI Dong-Hui,XU Jin-Gou,ZHANG Yi,ZHANG Chang-Gong.Fluorescence Immunoassay for HBsAg Using a Thermal Phase Separating Polymer[J].Chemical Research In Chinese Universities,1999,20(4):544-548. |
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| Authors: | ZHU Qing-Zhi YANG Huang-Hao LI Dong-Hui XU Jin-Gou ZHANG Yi ZHANG Chang-Gong |
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| Institution: | 1. The Research Laboratory of SEDC of Analytical Science for Material and Life Chemistry, Department of Chemistry, Xiamen University, Xiamen, 361005;
2. The Research Laboratory of SEDC of Analytical Science for Material and Life Chemistry Cancer Research Center, Xiamen University, Xiamen, 361005 |
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| Abstract: | A novel fluorescence immunoassay for HBsAg was developed by using polyNisopropylacrylamide(PNIP) as an immunoreaction carrier. In sandwich immunoassay, after two steps immunoreaction, PNIP precipitates out of water above a critical temperature of 31 , enabling a polymerbound immune complex to be separated from the solution. The calibration graph for HBsAg was linear over the range of 05100 g/mL with a detection limit of \{10 ng/mL\}. This method combines some advantages of both homogeneous and heterogeneous immunoassays, and has been applied to determining the HBsAg in human blood serum with satisfactory results. |
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| Keywords: | PolyNisopropylacrylamide Thermal phase separating technique Fluorescence immunoassay HBsAg |
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