| 化学-生物法制备D-精氨酸和L-瓜氨酸 |
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| 引用本文： | 刘毅,尹翠,缑灵山,朱炎,孙强.化学-生物法制备D-精氨酸和L-瓜氨酸[J].化学研究,2008,19(4):52-55. |
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| 作者姓名： | 刘毅 尹翠 缑灵山 朱炎 孙强 |
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| 作者单位： | 徐州医学院,药学系,江苏,徐州,221004 |
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| 基金项目： | 国家技术创新项目
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徐州医学院院长专项人才基金
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| 摘 要： | 在水溶液中以水杨醛为催化剂，L-精氨酸可以快速消旋为DL-精氨酸．后以DL-精氨酸为底物，利用粪链球菌（Streptococcus faecalis）精氨酸脱亚胺酶对L-精氨酸的专一脱亚胺作用，同时制备D精氨酸和L-瓜氨酸，分别以92．3％，94．2％的产率获得光学纯的D-精氨酸和L-瓜氨酸．
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| 关 键 词： | DL-精氨酸 D-精氨酸 L-瓜氨酸 化学消旋 精氨酸脱亚胺酶 酶法转化 |
Production of D-Arginine and L-Citrulline by Chemical Racemization and Enzymatic Conversion |
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| LIU Yi,YIN Cui,GOU Ling-shan,ZHU Yan,SUN Qiang.Production of D-Arginine and L-Citrulline by Chemical Racemization and Enzymatic Conversion[J].Chemical Research,2008,19(4):52-55. |
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| Authors: | LIU Yi YIN Cui GOU Ling-shan ZHU Yan SUN Qiang |
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| Institution: | (Department of Pharmacy, Xuzhou Medical College, Xuzhou 221004, Jiangsu, China) |
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| Abstract: | A practical method for the racemization of L-arginine has been developed.The whole process of racemization by this method can be completed within 6 h in the presence of 0.10 molar equivalent of salicylaldehyde.D-arginine and L-citrulline were produced by means of biotransformation of DL-arginine in the presence of arginine deiminase which was found in the Streptococcus faecalis.The yield of polarimeter analysis of purified D-arginine and L-citrulline was 92.3 % and 94.2 % respectively. |
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| Keywords: | DL-arginine D-arginine L-citrulline chemical racemization arginine deiminase enzymatic conversion |
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