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Direct electrochemistry of hemoglobin on graphene/Fe3O4 nanocomposite-modified glass carbon electrode and its sensitive detection for hydrogen peroxide |
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| Authors: | Yuqiao Wang Huijun Zhang Dan Yao Junjie Pu Yuan Zhang Xiaorui Gao Yueming Sun |
| Institution: | 1. School of Chemistry and Chemical Engineering, Southeast University, Nanjing, 211189, China 2. School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, 225002, China 3. School of Science, Hebei University of Engineering, Handan, 056038, China |
| Abstract: | Graphene/Fe3O4 nanocomposite was prepared for the immobilization of hemoglobin (Hb) to improve the electron transfer between Hb and glass carbon electrode (GCE). The characterization of nanocomposites was described by transmission electron microscopy, Fourier transform infrared, Raman spectroscopy, and X-ray photoelectron spectroscopy, respectively. The electrochemistry of Hb on the graphene/Fe3O4-based GCE was investigated by cyclic voltammetry and amperometric measurement. The modified electrode showed a wide linear range from 0.25 μmol/L to 1.7 mmol/L with a correlation coefficient of 0.9967. The detection limit of the H2O2 biosensor was estimated at 6.0?×?10?6?mol/L at a signal-to-noise ratio of 3. |
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