| 硫胺素-亚硝酸根体系的荧光性质研究及应用 |
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| 引用本文： | 李松青,李胜,黄灵芝,陈小明. 硫胺素-亚硝酸根体系的荧光性质研究及应用[J]. 分析试验室, 2001, 20(3): 23-24 |
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| 作者姓名： | 李松青 李胜 黄灵芝 陈小明 |
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| 作者单位： | 湘潭大学化学化工学院,;湘潭大学化学化工学院,;湘潭大学化学化工学院,;湘潭大学化学化工学院, |
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| 基金项目： | 湖南省自然科学基金资助项目 !(0 0 jjy2 0 84 ) |
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| 摘 要： | 研究了在碱性溶液中硫胺素（VB1）与亚硝酸根的荧光反应,建立了荧光光度法测定硫胺素的新方法。方法线性范围为1．28－324ng/mL,检出限0．52ng/mL,对15个浓度为100ng/mL的硫胺素溶液进行测定,相对标准偏差为1．4％。应用于药物中硫胺素的测定,回收率在96．5％－101．8％之间。
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| 关 键 词： | 硫胺素 亚硝酸根 荧光光度法 |
| 文章编号： | 1000-0720(2001)03-0023-02 |
Fluorescent property of thiamine-nitrite system and its application |
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| LI Song-qing,LI Sheng,HUANG Ling-zhi,Chen Xiao-ming. Fluorescent property of thiamine-nitrite system and its application[J]. Chinese Journal of Analysis Laboratory, 2001, 20(3): 23-24 |
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| Authors: | LI Song-qing LI Sheng HUANG Ling-zhi Chen Xiao-ming |
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| Abstract: | A new spectrofluorimetric method for determination of thiamine is reported. It is based on the oxidation of thiamine by nitrite in a basic medium to yield the fluorescent thiochrome. The fluorescence intensity is linear over the range of 1.28～324 ng/mL for thiamine with correlation coefficient of 0.9998. The detection limit is 0.52 ng/mL and the relative standard deviation for 100 ng/mL thiamine is 1.4%(n=15). The recoveries are between 96.5% and 101.8%. The method was applied to the determination of thiamine in commercial pharmaceutical preparations. |
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| Keywords: | Thiamine Nitrite Spectrofluorimetry |
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