A simple direct enzyme immunoassay of antibodies based on the differences in diffusion rates in a gel of a synthetic antigen and of its complex with antibodies |
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Authors: | Anatoly N Semenov Evgeny E Efremov |
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Institution: | (1) Russian-German Joint Venture Constanta, Gruzinsky per. 3/2, 123 056 Moscow, Russia;(2) National Cardiology Research Center, Moscow, Russia |
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Abstract: | A simple direct enzyme immunoassay for semiquantitative detec tion of antibodies is suggested. It is based on the difference
in diffusion rates in a gel for a synthetic low-mol-wt antigen and of its complexes with antibodies to be detected. Sensitivity
and specificity of the devel oped assay are equal to an ELISA method. The assay has been tested with antibodies against HIV
protein gp41 in rabbit serum. Possible applications and limitations of the method are discussed. |
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Keywords: | Enzyme immunoassay homogeneous immunoassay immunoassay in gel AIDS gp41 antibodies |
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