| 反相高效液相色谱法测定毛竹叶中总黄酮 |
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| 引用本文： | 张廷之,侯镜德,徐秀珠.反相高效液相色谱法测定毛竹叶中总黄酮[J].理化检验(化学分册),2001,37(3):117-118. |
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| 作者姓名： | 张廷之 侯镜德 徐秀珠 |
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| 作者单位： | 浙江大学分析测试中心 |
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| 摘 要： | 用反相高效液相色谱法，RP－C8柱为固定相，甲醇－水－冰乙酸（40＋60＋2）为流动相，以芦丁为参照物外标法定量，辅以二极管阵列检测技术，测定了毛竹叶中总黄酮的含量，并与光度法作了比较。
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| 关 键 词： | 反相高效液相色谱 毛竹叶 芦丁 参照物 二极管阵列 比色法 总黄酮 测定 |
| 文章编号： | 1001-4020(2001)03-0117-02 |
| 修稿时间： | 1999年8月16日 |
RPHPLC DETERMINATION OF TOTAL FLAVONOIDS IN PHYLLOSTACHYS HETEROCYCLA LEAF |
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| ZHANG Ting zhi,HOU Jing de,XU Xiu zhu.RPHPLC DETERMINATION OF TOTAL FLAVONOIDS IN PHYLLOSTACHYS HETEROCYCLA LEAF[J].Physical Testing and Chemical Analysis Part B:Chemical Analgsis,2001,37(3):117-118. |
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| Authors: | ZHANG Ting zhi HOU Jing de XU Xiu zhu |
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| Abstract: | The total flavonoids in phyllostachys heterocycla leaf is determined by using high performance liquid chromatography in which RP C 8 column is used as stationary phase, and the methanol water acetic acid (40+60+2) as mobile phase and diode array detecting technique is used. The contents of the total flavonoids in phyllostachys heterocycla leaf are determined by reference to standard compound rutin, and the results are compared with those obtained by photometry. |
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| Keywords: | Reversed phase high performance liquid chromatography Phyllostachys heterocycla leaf Flavonoids Diode array Photometry |
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