植物中鱼藤酮类化合物检测方法的改进

 现有分析鱼藤酮的高效液相方法是针对鱼藤酮的检测而建立的 ,因此它不适合于鱼藤酮类化合物的分析检测。比较鱼藤酮类化合物的UV吸收光谱发现 ,原方法中的检测波长 (2 80nm～ 30 0nm)不适于鱼藤素、毛鱼藤酮及其类似物的检测。通过试验确定适合鱼藤酮类化合物的检测波长为 2 40nm。用CHCl3 MeOH(体积比 9∶1)溶剂提取出的植物中的鱼藤酮类化合物 ,经C18柱进行分离后 ,以MeOH H2 O(体积比 6 6∶34 )为洗脱剂对鱼藤酮类化合物进行等度洗脱。实验结果表明 ,改进的方法可一次性分离检测出鱼藤酮、鱼藤素、毛鱼藤酮及其 12a 羟基 和 6a ,12a 脱氢-类似物，各峰分离良好。

Modification of the Analytical Method for Rotenoids in Plants

Rotenoids are the active ingredients of some botanical insecticides and prospective candidates as anticancer agents. The proper isolation and determination of rotenoids in plants is of great importance for their further research and development. However, the HPLC method available for this purpose was developed particularly for the detection and determination of rotenone, so it appears to be unsuitable for the analysis of other rotenoids such as deguelin, elliptone and their analogues. By checking the UV spectra, it has been found that four types of UV absorption patterns occurred among the major rotenoids isolated from the roots of Derris elliptica and leaves of Tephrosia vogelii, and that the detection wavelength at 240 nm is more adequate for the analysis of a complex of rotenoids than at 280 nm-300 nm, which is used for rotenone detection. The extraction of rotenoids from plants is conveniently carried out by CHCl3-MeOH(9:1, V/V) and the purification can be accomplished by filtration of the crude residue through a C18 reversed-phase cartridge. Rotenoids can be isocratically eluted by MeOH-H2O(66:34, V/V). The results showed that rotenone, deguelin, elliptone, and their 12a-hydroxy- and 6a,12a-dehydro-analogs can be easily detected by the modified method, along with a satisfactory peak separation. The rotenoid components might be characterized by their retention times and relative retention times based on rotenone, which were at a range of 3.26 min-39.42 min and 40.4%-489.1% respectively.