Capillary electrophoretic separation of enantiomers in a high-pH background electrolyte by means of the single-isomer chiral resolving agent octa(6-O-sulfo)-gamma-cyclodextrin

The new, alkali-stable, single-isomer, sulfated gamma-cyclodextrin, the sodium salt of octa(6-O-sulfo)-gamma-cyclodextrin (OS) was used for the first time to separate the enantiomers of non-ionic, acidic, basic and ampholytic analytes by capillary electrophoresis in high-pH aqueous background electrolytes. The effective mobilities and separation selectivities were found to follow trends similar to those observed earlier in acidic aqueous background electrolytes. OS proved to be a broadly applicable chiral resolving agent and afforded adequate peak resolution values with short separation times for a number of non-ionic, weak acid, weak base and ampholytic analytes.