| Characterization of Hapten-Protein Conjugates for Immunoassay of Polycyclic Aromatic Hydrocarbons（PAHs） |
| |
| 作者姓名： | ZHANG Yan-feng ;GAO Zhi-xian ;SUN Hong-wen ;DAI Shu-gui |
| |
| 作者单位： | [1]College of Environmental Science and Engineering; [2]Key Laboratory for Pollution Process and Environmental Criteria of Ministry of Education, Nankai University, Tianjin 300071, P R. China; [3]Institute of Hygiene and Environmental Medicine, Tianjin 300050, P R. China |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)
，
PhD Programs Foundation of Ministry of Education of China
|
| |
| 摘 要： | Preparation and characterization of the hapten-protein conjugates are fundamental to developing environmental immunoassays. As a hapten, 1-pyrenebutyric acid（PBA） was conjugated to the carrier protein of bovine serum albumin（BSA） or ovalbumin（OVA） by active ester method. Infrared spectra（IR） showed that PBA-BSA and PBA-OVA conjugates were successfully prepared. The number of the haptens conjugated to the carrier protein was determined by ultraviolet spectra（UV） and matrix-assisted laser desorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS）. The calculated average binding ratios of PBA/BSA and PBA/OVA were 18：1 and 10：1 by UV, and 31：1 and 22：1 by MALDI-TOF-MS, respectively. Although there was a discrepancy between the results determined by the two methods, both of them were useful for the characterization of the hapten-protein conjugates. The antibody was produced against the antigen of PBA-BSA, and the affinity was tested by the double agar diffusion method The conjugates and the antibody could be used for developing a sensitive and selective immunoassay of polycyclic aromatic hydrocarbons（PAHs）.
|
| 关 键 词： | 多轮芬芳烃 半抗原 结合物 免疫测定 |
| 收稿时间： | 13 November 2007 |
Characterization of Hapten-Protein Conjugates for Immunoassay of Polycyclic Aromatic Hydrocarbons(PAHs) |
| |
| ZHANG Yan-feng,;GAO Zhi-xian,;SUN Hong-wen,;DAI Shu-gui.Characterization of Hapten-Protein Conjugates for Immunoassay of Polycyclic Aromatic Hydrocarbons(PAHs)[J].Chemical Research in Chinese University,2008,24(6):697-700. |
| |
| Authors: | Yan-feng ZHANG Zhi-xian GAO Hong-wen SUN Shu-gui DAI |
| |
| Institution: | aCollege of Environmental Science and Engineering, Nankai University, Tianjin 300071, P. R. China;bKey Laboratory for Pollution Process and Environmental Criteria of Ministry of Education, Nankai University, Tianjin 300071, P. R. China;cInstitute of Hygiene and Environmental Medicine, Tianjin 300050, P. R. China |
| |
| Abstract: | Preparation and characterization of the hapten-protein conjugates are fundamental to developing environmental immunoassays.As a hapten,1-pyrenebutyric acid(PBA) was conjugated to the carrier protein of bovine serum albumin(BSA) or ovalbumin(OVA) by active ester method.Infrared spectra(IR) showed that PBA-BSA and PBA-OVA conjugates were successfully prepared.The number of the haptens conjugated to the carrier protein was determinedby ultraviolet spectra(UV) andmatrix-assistedlaser desorptionionization time-of-flightmass spectrometry(MALDI-TOF-MS).The calculated average binding ratios of PBA/BSA and PBA/OVA were 18:1 and 10:1 by UV,and 31:1 and 22:1 by MALDI-TOF-MS,respectively.Although there was a discrepancy between the results determined by the two methods,both of them were useful for the characterization of the hapten-protein conjugates.The antibody was produced against the antigen of PBA-BSA,and the affinity was tested by the double agar diffusion method.The conjugates and the antibody could be used for developing a sensitive and selective immunoassay of polycyclic aromatic hydrocarbons(PAHs). |
| |
| Keywords: | Polycyclic aromatic hydrocarbons Hapten Conjugate Immunoassay Characterization |
| 本文献已被 CNKI 维普 万方数据 ScienceDirect 等数据库收录！ |
