基于阳离子型共轭聚合物和核酸适体的腺苷检测新方法

建立了一种基于阳离子型共轭聚合物和核酸适体的腺苷检测新方法. 荧光素修饰的短链DNA与腺苷的核酸适体部分互补, 形成双链DNA; 阳离子型共轭聚合物通过静电作用与双链DNA结合, 发生高效率的荧光共振能量转移(FRET). 加入腺苷后, 腺苷与核酸适体发生特异性结合, 导致双链DNA分解成单链, 使静电吸引力下降, 能量转移效率降低. 通过阳离子型共轭聚合物对单双链DNA的高效识别, 可快速简易地检测出腺苷.

Novel Adenosine Detection Method Based on Cationic Conjugated Polymer and Aptamer

We designed a novel adenosine detection system based on cationic conjugated polymer (CCP) and aptamer. A fluorescein-modified short-chain DNA can partially hybridize with adenosine aptamer to form a double stand DNA(dsDNA). The CCP associated with the dsDNA by electrostatic attractions and an efficient fluorescence resonance energy transfer (FRET) from the CCP to the fluorescein occurred. When adenosine was added, it bonded specifically to the aptamer, making the dsDNA break into single stand DNA(ssDNA), then the FRET efficiency decreased. Thus, we could detect the adenosine quickly and easily through the high signal discrimination between dsDNA and ssDNA with the CCP. The results show that the degree of FRET efficiency decrease is related to the concentration of adenosine. This detection method has the advantages including easy operations, low costs, and relatively high sensitivity; moreover, it can be extended to detections of other targets owning aptamers.