| 电渗流与缓冲液浓度之间数学关系的线性表达 |
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| 引用本文： | 许旭,高建平,鲍笑岭.电渗流与缓冲液浓度之间数学关系的线性表达[J].中国化学,2006,24(1):85-88. |
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| 作者姓名： | 许旭 高建平 鲍笑岭 |
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| 作者单位： | [1]State Key Laboratory of Organometallic Chemistry, Laboratory of Analytical Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China [2]School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China |
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| 摘 要： | It was believed that electroosmotic mobility μeo is inversely proportional to the square root of the ionic strength L But the linear relationship for regression analysis was expressed differently in different papers. The paper studied the linear expression of the mathematical relationship between μeo and c （background buffer concentration） by mathematical transform and real experimental data.μeo values of fused silica capillary were determined in four buffer systems. Their experimental conditions were controlled carefully for decreasing temperature difference AT and pH difference ApH in 50 μm ID capillary, in which no double layer overlap existed. The linear relationship between the reciprocal of electroosmotic mobility and the square root of concentration （or ionic strength） was derived by mathematical method. The regression analysis of experimental data was shown to well correspond to the relationship. The constants in regression equation could be well defined and the calculated results were acceptable.
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| 关 键 词： | 电渗透法 缓冲器 硅石 化学反应 |
| 收稿时间： | 2005-02-23 |
| 修稿时间： | 2005-02-232005-09-15 |
Linear Expression of the Mathematical Relationship between Electroosmotic Mobility and Buffer Concentration |
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| Xu Xu;Gao JianPing;Bao XiaoLing.Linear Expression of the Mathematical Relationship between Electroosmotic Mobility and Buffer Concentration[J].Chinese Journal of Chemistry,2006,24(1):85-88. |
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| Authors: | Xu Xu;Gao JianPing;Bao XiaoLing |
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| Abstract: | It was believed that electroosmotic mobility μeo is inversely proportional to the square root of the ionic strength I. But the linear relationship for regression analysis was expressed differently in different papers. The paper studied the linear expression of the mathematical relationship between μeo and c (background buffer concentration) by mathematical transform and real experimental data. μeo values of fused silica capillary were determined in four buffer systems. Their experimental conditions were controlled carefully for decreasing temperature difference ΔT and pH difference ΔpH in 50 µm ID capillary, in which no double layer overlap existed. The linear relationship between the reciprocal of electroosmotic mobility and the square root of concentration (or ionic strength) was derived by mathematical method. The regression analysis of experimental data was shown to well correspond to the relationship. The constants in regression equation could be well defined and the calculated results were acceptable. |
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| Keywords: | capillary electrophoresis electroosmotic flow buffer concentration |
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