一种包覆型聚合物高效液相色谱固定相的制备与评价

当代应用最广的HPLC固定相中,硅胶基质填料化学稳定性较低,并且由于残存硅羟基而表现所谓第二效应,特别使碱性化合物的峰形拖尾;聚合物基质填料虽提高了化学稳定性,没有离子基团,但刚性降低,并且由于溶胀难于进行梯度淋洗;面包覆型聚合物填料则可综合二者的优点,本文对我们制备的这种固定相作初步报道。

Preparation and Evaluation of an Encapsulated Polymer HPLC Stationary Phase

A reversed phase stationary phase of HPLC made by covering a porous copolymer of ethyl-styrene with divinylbenzene on the surface of silica gel is reported. The encapsulated polymer packing material maintains the main advantages of both polymer and silica based material. it is not only chemically stable, ion-free, and not soluble in solvents, but also of high rigidity, and has compatibility for solvent changing from organic to aqueous and reverse. Such packing is easy to pack and has a high column efficiency. Its application to the separation of aniline derivatives is documented.