Determination of diagnostical markers of urolithiasis by capillary electrophoresis

Methods of the electrophoretic determination of the main markers of urolithiasis, i.e., inorganic cations, ammonium, potassium, sodium, magnesium, and calcium; inorganic anions, chloride, sulfate, and phosphate; and organic acid anions, urate, citrate and oxalate, and creatinine in 24-hour collection urine with spectrophotometric detection were developed. The detection limits of analytes ranged form 0.25–1.0 μg/mL. The precision and accuracy were 2–5% and 97–101.5%, respectively. In dependence on the analyte. The developed procedures were used in the analysis of 156 urine samples. Normal ranges were preliminarily determined on a group of healthy donors.