Quencher as leaving group: efficient detection of DNA-joining reactions |
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Authors: | Sando Shinsuke Kool Eric T |
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Institution: | Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA. |
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Abstract: | We describe a new fluorescence reporting strategy in which dabsyl, a well-known quencher, activates a hydroxyl group in a probe to convert it to a leaving group. When a nucleophilic phosphorothioate probe binds adjacent to a dabsyl quenched probe, autoligation occurs, releasing the quencher, and lighting up the probes, This signal change can be used to detect single nucleotide differences in DNA without enzymes or reagents. |
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