Hydride transfer versus hydrogen radical transfer in thymidylate synthase |
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Authors: | Hong Baoyu Haddad Majd Maley Frank Jensen Jan H Kohen Amnon |
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Institution: | Department of Chemistry, University of Iowa, Iowa City, Iowa 52242-1294, USA. |
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Abstract: | The nature of a H-transfer in the thymidylate synthase catalyzed reaction was investigated by comparison of the wild-type enzyme with the W80M mutant. The nature of the H-transfer was not affected, as indicated by intrinsic isotope effects and their temperature dependence. These findings support a single-step hydride transfer instead of a two-step radical transfer. |
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