| 硫普罗宁与牛血清白蛋白相互作用的研究 |
| |
| 引用本文： | 周能,周振. 硫普罗宁与牛血清白蛋白相互作用的研究[J]. 化学分析计量, 2011, 20(3): 53-56 |
| |
| 作者姓名： | 周能 周振 |
| |
| 作者单位： | 玉林师范学院化学与生物系,天然产物研究所,玉林537000 |
| |
| 基金项目： | 国家自然科学基金项目(81060360); 广西科学基金项目(2010GXNSFA013065); 广西教育厅科研项目(200911MS200) |
| |
| 摘 要： | 采用荧光技术研究了硫普罗宁与牛血清白蛋白(BSA)之间的结合作用,结果显示硫普罗宁对BSA的荧光猝灭为静态猝灭和动态猝灭共同作用的结果.经研究得到了反应的结合常数、结合热力学性质和结合位点等参数,确定了该药物与BSA之间的作用力类型为疏水作用.探究了金属离子和甘草次酸的共存对硫普罗宁与BSA结合的影响.
|
| 关 键 词： | 硫普罗宁 牛血清白蛋白 荧光光谱 甘草次酸 |
INTERACTION BETWEEN TIOPRONIN AND BOVINE SERUM ALBUMIN |
| |
| Zhou Neng,Zhou Zhen. INTERACTION BETWEEN TIOPRONIN AND BOVINE SERUM ALBUMIN[J]. Chemical Analysis And Meterage, 2011, 20(3): 53-56 |
| |
| Authors: | Zhou Neng Zhou Zhen |
| |
| Affiliation: | Zhou Neng,Zhou Zhen (Institute of Natural Products,Department of Chemistry & Biology,Yulin Teachers University,YuLin 537000,China) |
| |
| Abstract: | The interaction between tiopronin and bovine serum album(BSA) was investigated by fluorescence technique.The results demonstrated that the fluorescence quenching of BSA by tiopronin was a result of the combined action of static quenching and dynamic quenching.The association constants,thermodynamic property and the number of binding sites were obtained.The binding force between tiopronin and BSA was confirmed as hydrophobic interaction.The effect of metal ions and glycyrrhetinic acid on the binding between ... |
| |
| Keywords: | tiopronin bovine serum albumin fluorescence spectra glycyrrhetinic acid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
