人膀胱癌组织对不同波长的激光及其线偏振激光的衰减特性比较研究

采用双积分球系统和光辐射测量技术的基本原理以及运用生物组织的光学模型,研究了4765,488,4965,5145,532nm激光及其线偏振激光辐照人膀胱癌组织的光衰减特性。结果表明在5个不同的激光波长范围内,KubelkaMunktwoflux模型下的人膀胱癌组织对不同波长的激光及线偏振激光的衰减特性是不同的,人膀胱癌组织对4765,4965,532nm激光及其线偏振激光的总衰减系数或有效衰减系数均具有显著性差异(P<005),而对488,5145nm波长的激光及其线偏振激光的总衰减系数或有效衰减系数则均没有显著性差异(P>005),人膀胱癌组织对532nm激光及其线偏振激光的总衰减系数或有效衰减系数均明显大于其他4个波长的Ar 激光及其线偏振激光的总衰减系数或有效衰减系数。在传输理论下人膀胱癌组织对5个不同波长的激光及其线偏振激光的总衰减系数均随着波长的增大而减小,人膀胱癌组织对4765,4965,5145nm波长的激光及其线偏振激光的总衰减系数均有显著性差异(P<005),其对488,532nm波长的激光及其线偏振激光的总衰减系数均没有显著性差异(P>005)。

Comparative Research on Attenuation Characteristics of Human Bladder Cancer Tissue at Different Wavelengths of Laser and Their Linearly Polarized Laser in Vitro

In this paper, a double integrating spheres system, the basic principle of measuring technology of ray radiation, and the optical model of biological tissues were used for the study. Attenuation characteristics of human bladder cancer tissue at 467 5, 488, 496 5, 514 5 and 532 nm laser and their linearly polarized laser irradiation were studied. The results of measurement showed that the attenuation characteristics of human bladder cancer tissue in Kubelka Munk two flux model were different at different wavelengths of laser irradiation in the range of five different laser wavelengths. There were significant differences in the total attenuation or effective attenuation coefficients of human bladder cancer tissue at 476 5, 496 5, and 532 nm wavelengths of laser and their linearly polarized laser irradiation( P >0 05), and there were no significant differences at 488 and 514 5 nm wavelengths of laser and their linearly polarized laser irradiation in two flux model( P >0 05). The total attenuation and effective attenuation coefficients of human bladder cancer tissue at 532 nm laser and its linearly polarized laser irradiation were obviously bigger than those at other four different wavelengths of laser and their linearly polarized laser irradiation in two flux model. The total attenuation and effective attenuation coefficients of human bladder cancer tissue at five different wavelengths of laser and their linearly polarized laser irradiation increased with the decrease of these wavelengths, there were significant differences in those at 476 5, 496 5 and 514 5 nm wavelengths of laser and their linearly polarized laser irradiation( P <0 05), and there were no significant differences in those at 488 and 532 nm wavelengths of laser and their linearly polarized laser irradiation in light transport theory( P >0 05).