高效亲和色谱研究含有特定结构域肽与DNA的结合

高效亲和色谱研究含有特定结构域肽与ＤＮＡ的结合张若蘅，杨栩，李崇熙，徐筱杰（北京大学化学系北京１００８７１）１前言在脱氧核糖核酸（ＤＮＡ）结合蛋白质中，除了螺旋－转折。螺旋、锌指和亮氨酸拉链三种主要结构域之外￣［１］，近年来，一类含有β－转折结构的Ｓ...

A Study of the DNA-Binding Properties of Peptides Containing a Specified Motif by High Performance Affinity Chromatography(HPAC)

n contrast to many sequence-specific DNA binding proteins,for which DNA binding motifs such as thehelix-turn-helix,zinc-finger and leucinezipper have been well understood,a quite new mode of DNA bindingis identified as the S(T)PXX motif After proposing the role of a SPXXXGXP motif of peptides in DNA bind-ing process,we show here that the motif specified above prefers to recognize and bind to DNA with muchlower sequence specificity,using DNA cellulose AC. The novel results show that HPAc method is valuablefor producing accurate,highly reproducible features in elucidating the structural and molecular mechanisms ofnon-specific protein-DNA interaction.