| Institution: | 1. Technical University of Denmark, Department of Biotechnology and Biomedicine, Matematiktorvet 301, 2800, Kgs. Lyngby, Denmark;2. Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Aberdeen, AB24 3UE, Scotland, UK;3. Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven Campus, Gasthuisberg, O&N II Herestraat 49, Box 824, 3000, Leuven, Belgium;4. Marbio, UiT The Arctic University of Norway, Breivika, Tromsø, Norway;5. Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avenida del Conocimiento 34, Parque Tecnológico de Ciencias de la Salud, 18016, Armilla, Granada, Spain;6. CFisUC, Department of Physics, University of Coimbra, Rua Larga, 3004-516, Coimbra, Portugal |
| Abstract: | A new siderophore containing a 4,5-dihydroimidazole moiety was isolated from Pseudoalteromonas piscicida S2040 together with myxochelins A and B, alteramide A and its cycloaddition product, and bromo- and dibromoalterochromides. The structure of pseudochelin A was established by spectroscopic techniques including 2D NMR and MS/MS fragmentation data. In bioassays selected fractions of the crude extract of S2040 inhibited the opportunistic pathogen Pseudomonas aeruginosa. Pseudochelin A displayed siderophore activity in the chrome azurol S assay at concentrations higher than 50 μM, and showed weak activity against the fungus Aspergillus fumigatus, but did not display antibacterial, anti-inflammatory or anticonvulsant activity. |
