Electrospray mass spectrometry and supercritical fluid chromatography of methylated beta-cyclodextrins. |
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| Authors: | A Salvador B Herbreteau M Dreux |
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| Institution: | Institut de Chimie Organique et Analytique (ICOA), UPRES A, Université d'Orléans, France. |
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| Abstract: | Five commercial dimethylated beta-cyclodextrin (DM-beta-CD) samples were analysed by electrospray (or ionspray) mass spectrometry (ESI-MS) and supercritical fluid chromatography (SFC) with evaporative light scattering detection. A silica and a nitro-bonded silica were selected using CO2-methanol-acetonitrile-water and CO2-methanol as mobile phase, respectively. An extensive optimisation scheme was performed for mobile phase selection. Both SFC systems were used for analyses of complex DM-beta-CD samples. Peak identifications were made using off-line ESI-MS. Commercial DM-beta-CDs are impure mixtures of homologues and isomers and analysis reveals that every manufacturer produces a different mixture. |
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