| 丙三醇对水溶液中血红蛋白构象的影响——荧光猝灭和动态光散射研究 |
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| 引用本文： | 马林,魏志强,黄爱民,杨华,何维仁,林瑞森.丙三醇对水溶液中血红蛋白构象的影响——荧光猝灭和动态光散射研究[J].化学学报,2009,67(14):1566-1572. |
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| 作者姓名： | 马林 魏志强 黄爱民 杨华 何维仁 林瑞森 |
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| 作者单位： | (a广西大学化学化工学院 南宁 530004) (b浙江大学化学系 杭州 310027) |
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| 摘 要： | 利用荧光猝灭法和动态光散射法测定丙三醇-水混合溶剂中血红蛋白(Hb)与联苯胺的结合距离和Hb的流体动力学半径, 并通过分析Hb荧光光谱和吸收光谱的变化, 探讨丙三醇与蛋白质分子在水溶液中相互作用的机理及其对蛋白质构象的影响. 结果表明, 丙三醇-水混合溶剂中Hb通过优先水化作用形成更紧密的构象, 溶剂体系的氢键形成能力下降对稳定蛋白质的构象有重要的影响, 丙三醇浓度较高的混合溶剂中氢键网络发生崩塌, 导致蛋白质构象产生进一步的折叠. 实验显示, 尽管Hb在丙三醇-水混合溶剂中保持较完整的血红素疏水空穴结构, 但是血红素疏水空穴以外肽段的构象发生显著变化, 并对血红蛋白的聚集状态造成一定的影响.
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| 关 键 词： | 血红蛋白 丙三醇 构象 荧光猝灭 动态光散射 |
| 收稿时间： | 2008-10-17 |
| 修稿时间： | 2008-12-23 |
Influence of Glycerol on the Conformation of Hemoglobin in Aqueous Solutions: a Study of Fluorescence Quenching and Dynamic Light Scattering |
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| Ma Lin,Wei Zhiqiang,Huang Aimin,Yang Hua,He Weiren,Lin Ruisen.Influence of Glycerol on the Conformation of Hemoglobin in Aqueous Solutions: a Study of Fluorescence Quenching and Dynamic Light Scattering[J].Acta Chimica Sinica,2009,67(14):1566-1572. |
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| Authors: | Ma Lin Wei Zhiqiang Huang Aimin Yang Hua He Weiren Lin Ruisen |
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| Institution: | (a School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004) (b Department of Chemistry, Zhejiang University, Hangzhou 310027) |
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| Abstract: | The binding distance of benzidine to hemoglobin(Hb) and the hydrodynamic radii of Hb in glycerol-water mixtures were determined by a fluorescence quenching technique and dynamic light scattering measurements,respectively,and utilized to investigate the interaction between glycerol and the protein and its influence on the conformation of the protein in aqueous solution,together with the analysis of the fluorescence spectra and absorption spectra of Hb.The results suggested that glycerol promote a more compac... |
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| Keywords: | hemoglobin glycerol conformation fluorescence quenching dynamic light scattering |
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