氧化石墨烯修饰腐蚀型长周期光纤光栅的禽流感病毒免疫传感器

提出了一种使用基于氧化石墨烯修饰包层腐蚀型长周期光纤光栅应用于检测禽流感病毒的免疫传感器.氧化石墨烯通过氢键结合在包层腐蚀型长周期光纤光栅表面上,并通过共价键将禽流感病毒单克隆抗体与氧化石墨烯表面的羧基相结合.利用氧化石墨烯上吸附的禽流感病毒单克隆抗体与禽流感病毒抗原的特异性结合引起的长周期光纤光栅谐振波长变化进行检测.结果表明,该氧化石墨烯修饰包层腐蚀型长周期光纤光栅免疫传感器对禽流感病毒的检测极限为40 ng/mL,传感器的解离常数为~1.6×10^-7 mol/L,检测范围为40 ng/mL^200μg/mL.通过对禽流感病毒空白尿囊液、禽流感病毒尿囊液和新城疫病毒尿囊液进行检测,表明免疫传感器具有良好的特异性和临床性.该免疫传感器具有应用于禽流感病毒的快速和早期诊断的可能.

Avian Influenza Virus Immunosensor Based on Etched Long Period Fiber Grating Coated with Graphene Oxide

An immunosensor based on graphene oxide modified cladding etched long-period fiber grating sensor for detection of avian influenza virus is presented in this paper.Graphene oxide is coated on the surface of long-period fiber grating by hydrogen bonding using sodium hydroxide and the avian influenza virus monoclonal antibodies are combined with graphene oxide surface by a covalent bond.The resonant wavelength change of long-period fiber grating caused by the specific binding of avian influenza virus monoclonal antibody aolsorbed on graphene oxide to avian influenza virus antigen was used for detection.The experimental results show that the limit of detection of the graphene oxide modified cladding etched long-period fiber grating immunesensor can reach 40 ng/mL,the dissociation constant^1.6×10^-7 M and the detection range 40 ng/mL^200μg/mL.The detection results of avian influenza virus in avian influenza virus blank allantoic fluid,Avian influenza virus allantoic fluid and Newcastle disease virus allantoic fluid show that the immunosensor has good specificity and clinical characteristics.Therefore,the immunosensor could be potentially applied in the fast detection and early diagnosis of avian influenza virus.