Succinic Acid Production by Actinobacillus succinogenes Using Spent Brewer's Yeast Hydrolysate as a Nitrogen Source |
| |
Authors: | Min Jiang Kequan Chen Zhongmin Liu Ping Wei Hanjie Ying and Honam Chang |
| |
Institution: | (1) State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing, 210009, People’s Republic of China;(2) Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Republic of Korea |
| |
Abstract: | To develop a cost-effective fermentation medium, spent brewer's yeast hydrolysate was evaluated as a nitrogen source for succinic
acid production by Actinobacillus succinogenes NJ113 in glucose-containing media. Autolysis and enzymatic hydrolysis were used to hydrolyze the spent brewer's yeast cells
to release the nutrients. The results showed that enzymatic hydrolysis was a more effective method due to the higher succinic
acid yield and cell growth. However, the incomplete glucose consumption indicated existence of nutrient limitation. Vitamins
were subsequently identified as the main limiting factors for succinic acid production using enzymatically hydrolyzed spent
brewer's yeast as a nitrogen source. After the addition of vitamins, cell growth and succinic acid concentration both improved.
As a result, 15 g/L yeast extract could be successfully replaced with the enzymatic hydrolysate of spent brewer's yeast with
vitamins supplementation, resulting in a production of 46.8 g/L succinic acid from 68 g/L glucose. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|