离子色谱-荧光检测器同时测定多巴、肾上腺素和多巴胺

多巴、肾上腺素和多巴胺在流速为 1.0 m L·min-1,淋洗液为 0 .0 5mmol·L-1Na Cl的离子色谱仪上较好地分离 ,用激发波长为 2 80 nm、发射波长为 32 0 nm的荧光检测器进行测定 .多巴、肾上腺素和多巴胺的检测限分别为 1.53× 10 -6mol· L-1,8.11× 10 -7mol· L-1和 2 .95× 10 -7mol· L-1,具有良好的线性关系和重现性 .对药物样品与混合样品进行测定 ,结果令人满意

Simultaneous determination of dopa, epinephrine and dopamine with the ion chromatograph and fluorescent detector

Dopa, epinephrine and dopamine can be perfectly separated by the ion chromatograph with the eluent 0~{*1~}05 mmol/L NaCl at flow\|rate 1.0 mL/min, while the fluorescent detection is fixed at the excitation and emission wavelengths of 280nm and 320nm, respectively. The detection limits(S/N\=3) of dopa,epinephrine and dopamine are 1.5310\+\-7\ mol/L,8.1110\+{-7\ mol/L and 2.9510\+{-6\} mol/L, respectively. The method has been used to determine dopa, dopamine and epinephrine in medicine samples giving satisfactory results. Such as quite good linear relationship and repeatability.