Impact of lipid substitution on assembly and delivery of siRNA by cationic polymers |
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Authors: | Aliabadi Hamidreza Montazeri Landry Breanne Bahadur Remant K Neamnark Artphop Suwantong Orawan Uluda? Hasan |
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Institution: | Department of Chemical & Materials Engineering, Faculty of Engineering, University of Alberta, Edmonton, AB, Canada. |
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Abstract: | Characterization of a polymer library engineered to enhance their ability to protect and deliver their nucleotide cargo to the cells is reported. The ζ-potential continuously increased with higher polymer:siRNA weight ratio, and the ζ-potential of lipid-modified polymers:siRNA complexes were higher than PEI2 at all ratios. At polymer:siRNA ratio of 1:1, all lipid-substituted polymers showed complete protection against degradation. Lipid-modified polymers significantly increased the cellular uptake of siRNA complexes and down-regulation of GAPDH and P-gp (max. 66% and 67%, respectively). The results indicate that hydrophobic modification of low molecular PEI could render this otherwise ineffective polymer to a safe effective delivery system for intracellular siRNA delivery and protein silencing. |
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Keywords: | cancer down‐regulation polymers self‐assembly siRNA |
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