Chiral separation of N-(trans-4-isopropylcyclohexylcarbonyl)-D,L-phenylalanine isomers by high performance liquid chromatography期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Chiral separation of N-(trans-4-isopropylcyclohexylcarbonyl)-D,L-phenylalanine isomers by high performance liquid chromatography

Authors:	Gengliang Yang Zhiwei Li Dexian Wang Zhefeng Zhang Erdong Liu Yi Chen

Affiliation:	(1) College of Chemistry and Environmental Science, Hebei University, 071002 Baoding, P.R. China;(2) Center for Molecular Science, Institute of Chemistry, Chinese Academy of Science, 100080 Beijing, P.R. China

Abstract:	Summary A high-performance liquid chromatographic method was developed for the chiral separation of a new anti-diabetic agent, N-(trans-4-isopropylcyclohexylcarbonyl)-D-phenylalanine, and its L-enantiomer. The separation was performed on a Sumichiral OA-3300 column. Optimized mobile phase was 0.025 mol.L⁻¹ ammonium acetate in methanol solution. UV detection was at 210 nm. Baseline chiral separation was obtained within 12 minutes. The detection limits are 80 pg for the D-enantiomer and 120 pg for the L-enantiomer. RSD of the method was below 1% (n=5).

Keywords:	Column liquid chromatography Chiral separation N-(trans-4-isopropylcyclohexylcarbonyl)-D,L-phenylalanine isomers
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