Tyrosinase/laccase bienzyme biosensor for amperometric determination of phenolic compounds |
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| Authors: | Jolanta Kochana Pawel Nowak Anna Jarosz-Wilko&#x;azka Marta Biero&#x; |
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| Institution: | aFaculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland;bInstitute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, 30-239 Kraków, Poland;cDepartment of Biochemistry, Maria Curie-Sklodowska University, Sklodowska Square 3, 20-031 Lublin, Poland |
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| Abstract: | Tyrosinase/laccase bienzyme biosensor for amperometric determination of phenolic compounds was constructed. Enzymes were immobilized in titania gel matrix. The obtained biosensor was successfully used for determination of 2,6-dimethoxyphenol, 4-tertbutylcatechol, 4-methylcatechol, 3-chlorophenol and catechol. The highest sensitivity and the widest linear range were noticed for catechol, 234 mA L mol− 1 and 2.0 × 10− 7–3.2 × 10− 5 mol/L, respectively. Detection limit for catechol, at signal-to-noise ratio of 3 was 1.3 × 10− 7 mol/L. |
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| Keywords: | Tyrosinase Laccase Bienzyme biosensor Phenolic compounds Amperometric detection |
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