| Expression of Shiga Toxin B Subunit at Cell Surface in E. coli K-12 |
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| 作者姓名： | 苏国富 Himanshu N.Brahmbhatt Kenneth N.Timmis |
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| 作者单位： | Institute of Biotechnology，Academy of Military Medical Sciences CPLA，Beijing 100850，PRC，National Research Centre for Biotechnology，Germany，National Research Centre for Biotechnology，Germany |
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| 摘 要： | The three parts(Stx17B, Stx27B and StxB) of Shiga toxin B subunit have been fused into a cell surface exposed loop of the LamB protein at a BamH I site between residues 153 and 154. Western blotting revealed that the three parts of Shiga toxin B subunit could be expressed as the Lamb fusion proteins in E. coli. Indirect immunofluorescence and immunoelectron microscopy analyses showed fusion proteins LamB/Stx17B and LamB/Stx27B could be expressed at cell surface in E. coli, but fusion protein LamB/StxB could not be expressed at cell surface; it was aggregated in cytoplasm and was toxic to host. This expression system provided a new way to construct an oral live vaccine against Shigella dysenteriae 1.
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Expression of Shiga Toxin B Subunit at Cell Surface in E. coli K-12 |
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| SU Guo-Fu.Expression of Shiga Toxin B Subunit at Cell Surface in E. coli K-12[J].Science in China(Chemistry),1993(11). |
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| Authors: | SU Guo-Fu |
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| Abstract: | The three parts(Stx17B, Stx27B and StxB) of Shiga toxin B subunit have been fused into a cell surface exposed loop of the LamB protein at a BamH I site between residues 153 and 154. Western blotting revealed that the three parts of Shiga toxin B subunit could be expressed as the Lamb fusion proteins in E. coli. Indirect immunofluorescence and immunoelectron microscopy analyses showed fusion proteins LamB/Stx17B and LamB/Stx27B could be expressed at cell surface in E. coli, but fusion protein LamB/StxB could not be expressed at cell surface; it was aggregated in cytoplasm and was toxic to host. This expression system provided a new way to construct an oral live vaccine against Shigella dysenteriae 1. |
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| Keywords: | Shiga toxin B subunit LamB gene E coli K-12 cell surface expression |
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