| Highly sensitive potentiometric immunosensor for hepatitis B surface antigen diagnosis |
| |
| 作者姓名： | YUAN Ruo TANG Dianping CHAI Yaqin ZHANG Lingyan LIU Yan ZHONG Xia & DAI Jianyuan Chong Qing Key Laboratory of Analytical Chemistry College of Chemistry and Chemical Engineering Southwest China Normal Univer- sity Chongqing China |
| |
| 作者单位： | YUAN Ruo，TANG Dianping，CHAI Yaqin，ZHANG Lingyan，LIU Yan，ZHONG Xia & DAI Jianyuan Chong Qing Key Laboratory of Analytical Chemistry，College of Chemistry and Chemical Engineering，Southwest China Normal Univer- sity，Chongqing 400715，China |
| |
| 基金项目： | 国家自然科学基金，重庆市自然科学基金 |
| |
| 摘 要： | Immunosenors are of great interest because oftheir potential utility as specific, simple, label-free anddirect detection techniques and reductions in size, costand time of analysis compared with conventional im-munoassay techniques. The immunoassays with …
|
| 收稿时间： | 21 April 2009 |
Highly sensitive potentiometric immunosensor for hepatitis B surface antigen diagnosis |
| |
| YUAN Ruo,TANG Dianping,CHAI Yaqin,ZHANG Lingyan,LIU Yan,ZHONG Xia & DAI Jianyuan Chong Qing Key Laboratory of Analytical Chemistry,College of Chemistry and Chemical Engineering,Southwest China Normal Univer- sity,Chongqing ,China.Highly sensitive potentiometric immunosensor for hepatitis B surface antigen diagnosis[J].Science in China(Chemistry),2005,48(1):49-57. |
| |
| Authors: | Yuan Ruo TANG Dianping CHAI Yaqin ZHANG Lingyan LIU Yan ZHONG Xia DAI Jianyuan |
| |
| Institution: | Chong Qing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing 400715, China |
| |
| Abstract: | A highly sensitive potentiometric immunosensor for the diagnoses of epidemic diseases has been developed by means of self-assembly
to immobilize hepatitis B surface antibody (HBsAb) for the detection of hepatitis B surface antigen (HBsAg) as a model. At
first, the Nafion containing ™SO
3
™
groups was immobilized on a platinum electrode surface to absorb the ™NH3
+ groups of antibody moleculesvia the opposite-charged adsorption technique, in the meantime, hepatitis B surface antibodies were adsorbed onto the surface
of Au nanoparticles, then hepatitis B surface antibodies and Au nanopartilces were entrapped into polyvinyl butyral on the
surface of Nafion film. The modified procedure was further characterized by electrochemical impedance spectroscopy (EIS) and
cyclic voltammetry (CV). The influence and factors influencing the performance of resulting immunosensor were studied in detail.
The resulting immunosensor exhibited sigmoid curve with log HBsAg concentrations, high sensitivity, wide linear range from
26 to 1280 ng · mL−1 with a detection limit of 3.1 ng · mL−1, rapid potentiometric response (σ min) and long-term stability (/s>4 months). Analytical results of clinical samples show
that the developed immunoassay is comparable with the enzyme-linked immunosorbent assays (ELISAs) method, implying a promising
alternative approach for detecting HBsAg in the clinical diagnosis. |
| |
| Keywords: | nafion polyvinyl butyral colloidal Au hepatitis B potentiometric immunosensor platinum electrode |
| 本文献已被 CNKI 万方数据 SpringerLink 等数据库收录！ |
