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Evaluation of cellulase preparations for hydrolysis of hardwood substrates |
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| Authors: | Alex Berlin Neil Gilkes Douglas Kilburn Vera Maximenko Renata Bura Alexander Markov Anton Skomarovsky Alexander Gusakov Arkady Sinitsyn Oleg Okunev Irina Solovieva John N Saddler |
| Institution: | 1. Forest Products Biotechnology, Faculty of Forestry, The University of British Columbia, V6T 1Z4, Vancouver, BC, Canada 2. Department of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University, Vorobyevy Gory, 119899, Moscow, Russian Federation 3. Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, 142292, Moscow Region, Russian Federation |
| Abstract: | Seven cellulase preparations from Penicillium and Trichoderma spp. were evaluated for their ability to hydrolyze the cellulose fraction of hardwoods (yellow poplar and red maple) pretreated by organosolv extraction, as well as model cellulosic substrates such as filter paper. There was no significant correlation among hydrolytic performance on pretreated hardwood, based on glucose release, and filter paper activity. However, performance on pretreated hardwood showed significant correlations to the levels of endogenous β-glucosidase and xylanase activities in the cellulase preparation. Accordingly, differences in performance were reduced or eliminated following supplementation with a crude β-glucosidase preparation containing both activities. These results complement a previous investigation using softwoods pretreated by either organosolv extraction or steam explosion. Cellulase preparations that performed best on hardwood also showed superior performance on the softwood substrates. |
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