HPLC-ESI-TOFMS检测尿液中的雌二醇

用HPLC—ESI-TOFMS建立了快速灵敏检测尿中雌二醇的方法，尿样中雌二醇经盐酸水解后用OASISHLB小柱萃取．色谱柱为XTerraMS C18反相分离柱（3．5μm，2．1mm×100mm），流动相为水和乙腈，梯度洗脱，流速为0．2mL／min，紫外检测波长为280nm，外标法定量．质谱采用负离子电离模式．线性方程：y=1．79×10^5x＋2．59×10^4，线性范围：0．0328—32．8mg／L，线性相关系数R^2=0．9979，最小检出浓度0．00328mg／L，萃取回收率101．83％．应用本方法对10例正常人尿样进行测定，结果满意．相比较其它检测尿中雌二醇的方法来说，此法比较简单、快速、准确，为临床的诊断提供有价值的指标．

Detection of Estradiol in Urine by HPLC-ESI-TOFMS

By using HPLC-ESI-TOF MS,we established a convenient and rapid method for detecting estradiol in human urine.The target compounds in urine were firstly hydrolyzed in HCl medium and extracted by OASIS HLB columns.The separation was carried out on a XTerraMS C18(3.5 μm,2.1 mm×100 mm) RP column at 35 ℃ with a linear gradient elution of water(A)-acetonitrile(B).The flow-rate of 0.2 mL/min and UV detection at 280 nm were used in this study.The Mass was in negative mode.The limit of detection and the linear range of estradiol were 0.003 28mg/L and 0.032 8～32.8 mg/L respectively,with extraction recovery rate of 101.83 %.This method has been applied to the determination of estriol in 10 human urine samples with satisfactory results.