3-H-YDROXYRETINAL AS A CHROMOPHORE OF Drosophila melanogaster VISUAL PIGMENT ANALYZED BY HIGH-PRESSURE LIQUID CHROMATOGRAPHY |
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| Authors: | Teiichi Tanimura Kunio Isono Yasuo Tsukahara |
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| Institution: | Division of Behavior and Neurobiology, National Institute for Basic Biology, Okazaki, 444 Japan;Research Center for Applied Information Sciences, Tohoku University, Katahira, Sendai, 980 Japan |
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| Abstract: | Abstract— The chromophore of the visual pigment of the fruit fly Drosophila melanogaster was studied by high-pressure liquid chromatography (HPLC). Oxime derivatives of the chromophore were successfully analyzed with a more polar solvent than has been used for retinaloximes. A preceding monochromatic irradiation resulted in reversible interchanges in the amount among geometric isomers of the oximes that reflected the photointerconversion of rhodopsin and metarhodopsin of the peripheral photoreceptors (Rl-R6). Authentic all- trans -3-h-ydroxyretinal was used to identify the chromophore from metarhodopsin. Results demonstrated that the chromophore of the visual pigment in Drosophila is 3-h-ydroxyretinal as was proposed in larger flies. |
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