Label-free electrochemical detection of DNA hybridization on gold electrode |
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Institution: | 1. Institute of Engineering Thermophysics, Chinese Academy of Sciences, Beijing 100190, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;1. Department of Chemistry, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran;2. School of Chemistry, University of Melbourne, Victoria 3010, Australia;1. Institute for High Pressure Physics, Russian Academy of Sciences, Troitsk, 108840, Moscow, Russia;2. Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region 141700, Russia;1. BAE Laboratory, Université de Perpignan Via Domitia, Perpignan, France;2. Department of Chemistry, National Institute of Technology, Warangal, India;3. Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology (CIIT), Lahore, Pakistan |
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Abstract: | A label-free electrochemical detection protocol for DNA hybridization is reported for the first time by using a gold electrode (AuE). The oxidation signal of guanine was monitored at +0.73 V by using square wave voltammetry (SWV) on self-assembled l-cysteine monolayer (SAM) modified AuE. The electrochemical determination of hybridization between an inosine substituted capture probe and native target DNA was also accomplished. 6-mer adenine probe was covalently attached to SAM via its amino link at 5′ end. Then, 6-mer thymine-tag of the capture probe was hybridized with the adenine probe, thus left the rest of the oligonucleotide available for hybridization with the target. The dependence of the guanine signal upon the concentration of the target was observed. Probe modified AuE was also challenged with non-complementary and mismatch containing oligonucletides. Label-free detection of hybridization on AuE is greatly advantageous over the existing carbon and mercury electrode materials, because of its potential applicability to microfabrication techniques. Performance characteristics of the genosensor are described, along with future prospects. |
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