Chemiluminescence determination of 20 amino acids and the mechanism study

It was found that 20 amino acids could inhibit the intensity of the luminol-H2O2-CuSO4 chemiluminescence system. Using this character, a rapid and sensitive method for the determination of 20 amino acids was developed with flow-injection coupled with chemiluminescence detection. Under the optimal conditions, the detection limits of 20 amino acids were in the range of 4.5 × 10–7 to 4.3 × 10–10 mol/L, and the relative standard deviations were less than 3.2%. The proposed method was successfully applied to drug analysis. The possible mechanism was also discussed.